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Some DNA Dismissed As ‘Junk’ Is Crucial To Embryo Development

By: Richard Harris | NPR
Posted on: Thursday, June 21, 2018

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One of the enduring mysteries of biology is why so much of the DNA in our chromosomes appears to be simply junk. In fact, about half of the human genome consists of repetitive bits of DNA that cut and paste themselves randomly into our chromosomes, with no obvious purpose.

A study published Thursday finds that some of these snippets may actually play a vital role in the development of embryos.

The noted biologist Barbara McClintock, who died in 1992, discovered these odd bits of DNA decades ago in corn, and dubbed them “jumping genes.” (She won a Nobel prize for that finding in 1983.) McClintock’s discovery stimulated generations of scientists to seek to understand this bizarre phenomenon.

Some biologists have considered these weird bits of DNA parasites, since they essentially hop around our chromosomes and infect them, sometimes disrupting genes and leaving illness in their wake. But Miguel Ramalho-Santos, a biologist at the University of California, San Francisco, doesn’t like that narrative.

“It seemed like a waste of this real estate in our genome — and in our cells — to have these elements and not have them there for any particular purpose,” Ramalho-Santos says. “So we just asked a very simple question: Could they be doing something that’s actually beneficial?”

He and his colleagues focused on a jumping gene called LINE-1; all told, copies of it make up a whopping 20 percent of our entire DNA. Ramalho-Santos’ lab studies embryos, so the team wondered whether LINE-1 played any role in prompting a single fertilized egg to develop into an embryo.

Normally, when biologists want to study one bit of a cell’s genetic material, they find a way to eliminate it to see how the cell behaves in its absence. That’s impossible to do in the case of the LINE-1 genes, since that would mean editing out 20 percent of the entire genome, notes postdoctoral researcher Michelle Percharde.

Instead, the scientists devised a way to silence this abundant DNA inside the cells of a mouse embryo to see what would happen.

“What we found was that, instead of it being good for the cells,” she says, “the cells did very poorly.”

In a series of experiments published in the journal Cell, the scientists conclude that LINE-1 seems to be essential in the earliest stages of an embryo’s development.

“That’s a very key role,” says Ramalho-Santos, “because, as you can imagine, if you can’t make a tiny little embryo — if you can’t make embryonic stem cells, which are the cells that give rise to the entire body — you don’t have any body.”

His team’s discovery hinged in part on developing new lab techniques, but Ramalho-Santos says that’s not all.

“I think the biggest advancement here was actually a mentality shift.”

What’s been commonly dismissed as “junk DNA” does clutter the genome, and it’s clear that it can also cause harm. But Ramalho-Santos argues that biologists should also think harder about its function.

Nels Elde, an evolutionary biologist at the University of Utah, agrees — up to a point.

“I might be a little old-fashioned, but I’m still holding onto this notion of junk DNA,” Elde says. “Think of it this way. If you come into my office and look at the desk, you’ll look at it and say, ‘This thing is strewn with junk.’ And I may say to you, ‘Actually that’s a very customized filing system that you’re looking at.’ In fact, I think we’re both right.”

Elde believes that these repetitive bits of DNA (known technically as transposable elements) exist primarily because they cut and paste themselves throughout the genome. They reproduce. And in that sense they are parasites.

Sometimes they cause disease by disrupting the DNA where they insert themselves. But sometimes the mutations they cause can lead, for example, to new varieties of crops and new breeds of dogs.

“You can make massive genetic jumps — just in single events — that can really change the course of an entire species,” Elde says.

From the point of view of an evolutionary biologist, that’s enough reason for them to exist. But it’s increasingly clear that there’s even more to the story of these jumping genes.

“These things may be repurposed” to perform other functions in the cell, Elde says. “And that’s what we’re starting to discover, and that’s what [the new research] paper begins to tackle as well.”

Ramalho-Santos is pursuing these questions as he moves his lab to the University of Toronto, and Percharde says she plans to keep exploring these questions, as well. Her postdoctoral fellowship is now at an end, so she’s moving back to London to continue her research career.

The two are not alone in finding this a fruitful line of research. Elde says this idea is starting to gain momentum, as scientists figure out new ways to explore this fascinating realm of biology.